Konecka Edyta, Baranek Jakub, Hrycak Anita, Kaznowski Adam
Department of Microbiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
ScientificWorldJournal. 2012;2012:710501. doi: 10.1100/2012/710501. Epub 2012 May 1.
We attempted to search novel Bacillus thuringiensis strains that produce crystals with potential utility in plant protection and with higher activity than strains already used in biopesticide production. Seven B. thuringiensis soil and water isolates were used in the research. We predicted the toxicity of their crystals by cry gene identification employing PCR method. The isolate MPU B63 with interesting, according to us, genes content was used in evaluating its crystal toxicity against Cydia pomonella caterpillars. The strain MPU B63 was cultured from water sample and had cry1Ab, cry1B, and cry15 genes. The LC₅₀ crystals of MPU B63 were compared to LC₅₀ of commercial bioinsecticide Foray determined against C. pomonella (codling moth). The activity of MPU B63 inclusions against codling moth larvae was approximately 24-fold higher than that of Foray. The results are a promising introduction for further study evaluating the potential usefulness of isolate MPU B63 crystals in plant protection.
我们试图寻找新型苏云金芽孢杆菌菌株，这些菌株产生的晶体在植物保护方面具有潜在用途，且活性高于生物农药生产中已使用的菌株。本研究使用了7株苏云金芽孢杆菌土壤和水分离株。我们通过采用PCR方法进行cry基因鉴定来预测其晶体的毒性。分离株MPU B63具有我们认为有趣的基因组成，用于评估其晶体对苹果蠹蛾幼虫的毒性。菌株MPU B63从水样中培养得到，具有cry1Ab、cry1B和cry15基因。将MPU B63的晶体半数致死浓度（LC₅₀）与针对苹果蠹蛾测定的商业生物杀虫剂Foray的LC₅₀进行比较。MPU B63内含物对苹果蠹蛾幼虫的活性比Foray高约24倍。这些结果为进一步研究评估分离株MPU B63晶体在植物保护中的潜在用途提供了有前景的开端。
