Ibarra Jorge E, del Rincón M Cristina, Ordúz Sergio, Noriega David, Benintende Graciela, Monnerat Rose, Regis Leda, de Oliveira Cláudia M F, Lanz Humberto, Rodriguez Mario H, Sánchez Jorge, Peña Guadalupe, Bravo Alejandra
CINVESTAV IPN, Irapuato 36500, Guanajuato, México.
Appl Environ Microbiol. 2003 Sep;69(9):5269-74. doi: 10.1128/AEM.69.9.5269-5274.2003.
The characterization of selected Bacillus thuringiensis strains isolated from different Latin America countries is presented. Characterization was based on their insecticidal activity against Aedes aegypti, Culex quinquefasciatus, and Anopheles albimanus larvae, scanning electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and plasmid profiles as well as PCR analysis using novel general and specific primers for cry and cyt genes encoding proteins active against mosquitoes (cyt1, cyt2, cry2, cry4A, cry4B, cry10, cry11, cry17, cry19, cry24, cry25, cry27, cry29, cry30, cry32, cry39, and cry40). Strains LBIT315, LBIT348, and IB604 showed threefold higher mosquitocidal activity against A. aegypti and C. quinquefasciatus larvae than B. thuringiensis subsp. israelensis and displayed high similarities with the B. thuringiensis subsp. israelensis used in this study with regard to protein and plasmid profiles and the presence of cry genes. Strain 147-8906 has activity against A. aegypti similar to that of B. thuringiensis subsp. israelensis but has different protein and plasmid profiles. This strain, harboring cry11, cry30, cyt1, and cyt2 genes, could be relevant for future resistance management interventions. Finally, the PCR screening strategy presented here led us to identify a putative novel cry11B gene.
本文介绍了从不同拉丁美洲国家分离出的部分苏云金芽孢杆菌菌株的特性。特性鉴定基于它们对埃及伊蚊、致倦库蚊和白纹伊蚊幼虫的杀虫活性、扫描电子显微镜观察、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳、质粒图谱分析，以及使用针对编码对蚊子有活性的蛋白质的cry和cyt基因（cyt1、cyt2、cry2、cry4A、cry4B、cry10、cry11、cry17、cry19、cry24、cry25、cry27、cry29、cry30、cry32、cry39和cry40）的新型通用引物和特异性引物进行的PCR分析。菌株LBIT315、LBIT348和IB604对埃及伊蚊和致倦库蚊幼虫的杀蚊活性比苏云金芽孢杆菌以色列亚种高三倍，并且在蛋白质和质粒图谱以及cry基因的存在方面与本研究中使用的苏云金芽孢杆菌以色列亚种高度相似。菌株147 - 8906对埃及伊蚊的活性与苏云金芽孢杆菌以色列亚种相似，但蛋白质和质粒图谱不同。该菌株携带cry11、cry30 c、yt1和cyt2基因，可能与未来的抗性管理干预措施相关。最后，本文介绍的PCR筛选策略使我们鉴定出一个推定的新型cry11B基因。
