Insect Pathology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Appl Environ Microbiol. 1989 Oct;55(10):2437-42. doi: 10.1128/aem.55.10.2437-2442.1989.
We found the insect control agent Bacillus thuringiensis to be a ubiquitous soil microorganism. Using acetate selection to screen soil samples, we isolated B. thuringiensis in 785 of 1,115 soil samples. These samples were obtained in the United States and 29 other countries. A total of 48% of the B. thuringiensis isolates (8,916 isolates) fit the biochemical description of known varieties, while 52% represented undescribed B. thuringiensis types. Over 60% (1,052 isolates) of the isolates tested for toxicity were toxic to insects in the orders Lepidoptera or Diptera. Soil samples were collected from various habitats, including those habitats with different numbers of insects. The current presence of insects did not predict the presence of B. thuringiensis in a particular soil sample. B. thuringiensis was most abundant in samples from Asia.
我们发现苏云金芽孢杆菌是一种普遍存在的土壤微生物。使用醋酸盐选择法筛选土壤样本，我们从 1115 个土壤样本中的 785 个样本中分离出苏云金芽孢杆菌。这些样本来自美国和其他 29 个国家。在 48%的苏云金芽孢杆菌分离株（8916 株）中，符合已知变种的生化描述，而 52%则代表未描述的苏云金芽孢杆菌类型。在测试毒性的分离株中，超过 60%（1052 株）对鳞翅目或双翅目昆虫具有毒性。土壤样本取自各种生境，包括昆虫数量不同的生境。昆虫的当前存在并不能预测特定土壤样本中苏云金芽孢杆菌的存在。苏云金芽孢杆菌在亚洲的样本中最为丰富。
