Lee Sei-Jung, Lim Kye-Taek
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, 300 Yongbong-Dong, Kwang-ju, 500-757, South Korea.
J Biomed Sci. 2008 Jan;15(1):111-21. doi: 10.1007/s11373-007-9196-9. Epub 2007 Jul 27.
This study was carried out to investigate the chemopreventive effects of glycoprotein (UDN glycoprotein, 116-kDa) isolated from Ulmus davidiana Nakai on colitis-mediated colorectal cancer (CRC) in A/J mice. UDN glycoprotein intake significantly reduced the incidence and the multiplicity of colorectal tumors, induced by combination treatment with 10 mg/kg 1,2-dimethylhydrazine (DMH) and 2% dextran sodium sulfate (DSS). We found that the abnormal levels of lactate dehydrogenase (LDH), thiobarbituric acid reactive substances (TBARS), and nitric oxide (NO) were significantly suppressed in proportion to the concentration of UDN glycoprotein (0.01% and 0.02%) in the mice serum. In addition, consumption of UDN glycoprotein attenuated the activities of proliferating cell nuclear antigen (PCNA), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), and inhibited the DNA-binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the mice colonic tissue. Interestingly, the results obtained from reverse transcription polymerase chain reaction (RT-PCR) assay showed that 0.02% UDN glycoprotein inhibited the expressions of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 mRNA in the mice. Collectively, these results suggest that UDN glycoprotein has chemopreventive activity via modulation of inflammation-related factors responsible for development of colitis-mediated CRC in A/J mice.
本研究旨在探讨从朝鲜榆中分离出的糖蛋白（UDN糖蛋白，116 kDa）对A/J小鼠结肠炎介导的结直肠癌（CRC）的化学预防作用。摄入UDN糖蛋白显著降低了由10 mg/kg 1,2 - 二甲基肼（DMH）和2%葡聚糖硫酸钠（DSS）联合处理诱导的结直肠肿瘤的发生率和多发性。我们发现，小鼠血清中乳酸脱氢酶（LDH）、硫代巴比妥酸反应性物质（TBARS）和一氧化氮（NO）的异常水平与UDN糖蛋白浓度（0.01%和0.02%）成比例地显著降低。此外，摄入UDN糖蛋白减弱了增殖细胞核抗原（PCNA）、诱导型一氧化氮合酶（iNOS）和环氧化酶 - 2（COX - 2）的活性，并抑制了小鼠结肠组织中核因子 - κB（NF - κB）和活化蛋白 - 1（AP - 1）的DNA结合活性。有趣的是，逆转录聚合酶链反应（RT - PCR）分析结果表明，0.02%的UDN糖蛋白抑制了小鼠肿瘤坏死因子（TNF） - α和白细胞介素（IL） - 6 mRNA的表达。总体而言，这些结果表明UDN糖蛋白通过调节与A/J小鼠结肠炎介导的CRC发生相关的炎症相关因子而具有化学预防活性。
