Lee Sei-Jung, Lim Kye-Taek
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, Kwangju 500-757, Republic of Korea.
Biomed Pharmacother. 2009 Aug;63(7):528-36. doi: 10.1016/j.biopha.2008.08.015. Epub 2008 Sep 16.
The purpose of this study is to investigate the anti-inflammatory potentials of a 24-kDa glycoprotein isolated from Zanthoxylum piperitum DC fruit (ZPDC glycoprotein) in dextran sulfate sodium (DSS)-stimulated ICR mouse colitis. ZPDC glycoprotein was administered to mice at 10 and 20 mg/kg for 7 days and then the mice were co-treated with 5% DSS for another 7 days in presence of ZPDC glycoprotein and killed on day 15. The results showed that ZPDC glycoprotein has inhibitory effects on levels of disease activity index and large intestine shortening in DSS-treated mice. In addition, ZPDC glycoprotein suppresses the formation of thiobarbituric acid reactive substances, production of inducible nitric oxide, and release of lactate dehydrogenase in DSS-treated mice plasma. Interestingly, we found that consumption of ZPDC glycoprotein (20 mg/kg) significantly inhibited the expressions of interleukin-1beta and tumor necrosis factor-alpha, inducible nitric oxide synthase, and cyclooxygenase-2 via modulation of transcriptional activity of nuclear factor-kappa B in DSS-treated mice colon. Collectively, these results suggest that ZPDC glycoprotein is useful for prevention of inflammatory gastrointestinal diseases.
本研究旨在探讨从花椒果实中分离出的一种24 kDa糖蛋白（ZPDC糖蛋白）在葡聚糖硫酸钠（DSS）诱导的ICR小鼠结肠炎中的抗炎潜力。将ZPDC糖蛋白以10和20 mg/kg的剂量给予小鼠，持续7天，然后在ZPDC糖蛋白存在的情况下，用5% DSS对小鼠进行联合处理，持续7天，并在第15天处死小鼠。结果表明，ZPDC糖蛋白对DSS处理的小鼠的疾病活动指数水平和大肠缩短具有抑制作用。此外，ZPDC糖蛋白可抑制DSS处理的小鼠血浆中硫代巴比妥酸反应性物质的形成、诱导型一氧化氮的产生以及乳酸脱氢酶的释放。有趣的是，我们发现摄入ZPDC糖蛋白（20 mg/kg）可通过调节DSS处理的小鼠结肠中核因子-κB的转录活性，显著抑制白细胞介素-1β、肿瘤坏死因子-α、诱导型一氧化氮合酶和环氧化酶-2的表达。总体而言，这些结果表明ZPDC糖蛋白可用于预防炎症性胃肠道疾病。
