Rochat Tatiana, Bermúdez-Humarán Luis, Gratadoux Jean-Jacques, Fourage Christel, Hoebler Christine, Corthier Gérard, Langella Philippe
Unité d'Ecologie et Physiologie du Système Digestif, Centre de Recherche INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
Microb Cell Fact. 2007 Jul 20;6:22. doi: 10.1186/1475-2859-6-22.
Human immune cells generate large amounts of reactive oxygen species (ROS) throughout the respiratory burst that occurs during inflammation. In inflammatory bowel diseases, a sustained and abnormal activation of the immune system results in oxidative stress in the digestive tract and in a loss of intestinal homeostasis. We previously showed that the heterologous production of the Lactobacillus plantarum ATCC14431 manganese-dependant catalase (MnKat) in Lb. casei BL23 successfully enhances its survival when exposed to oxidative stress. In this study, we evaluated the preventive effects of this antioxidative Lb. casei strain in a murine model of dextran sodium sulfate (DSS)-induced moderate colitis.
Either Lb. casei BL23 MnKat- or MnKat+ was administered daily to mice treated with DSS for 10 days. In contrast to control mice treated with PBS for which DSS induced bleeding diarrhea and mucosal lesions, mice treated with both Lb. casei strains presented a significant (p < 0.05) reduction of caecal and colonic inflammatory scores.
No contribution of MnKat to the protective effect from epithelial damage has been observed in the tested conditions. In contrast, these results confirm the high interest of Lb. casei as an anti-inflammatory probiotic strain.
人类免疫细胞在炎症期间发生的呼吸爆发过程中会产生大量活性氧（ROS）。在炎症性肠病中，免疫系统的持续异常激活会导致消化道氧化应激和肠道内稳态丧失。我们之前表明，在干酪乳杆菌BL23中异源表达植物乳杆菌ATCC14431的锰依赖性过氧化氢酶（MnKat），在暴露于氧化应激时能成功提高其存活率。在本研究中，我们评估了这种具有抗氧化作用的干酪乳杆菌菌株在葡聚糖硫酸钠（DSS）诱导的中度结肠炎小鼠模型中的预防效果。
将干酪乳杆菌BL23 MnKat - 或MnKat + 每天给予用DSS处理10天的小鼠。与用PBS处理的对照小鼠相比，DSS诱导其出现出血性腹泻和粘膜损伤，而用两种干酪乳杆菌菌株处理的小鼠盲肠和结肠炎症评分显著降低（p < 0.05）。
在测试条件下未观察到MnKat对上皮损伤保护作用的贡献。相反，这些结果证实了干酪乳杆菌作为抗炎益生菌菌株具有很高的研究价值。
