Gradaschi Victoria, Payaslian Florencia, Dieterle Maria Eugenia, Rondón Salazar Liliana, Urdániz Estefanía, Di Paola Matias, Peña Cárcamo José, Zon Fabio, Allievi Mariana, Sosa Ezequiel, Fernandez Do Porto Darío, Dunne Matthew, Goeller Pauline, Klumpp Jochen, Raya Raúl Ricardo, Reyes Alejandro, Piuri Mariana
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Phage (New Rochelle). 2021 Mar 1;2(1):57-63. doi: 10.1089/phage.2020.0029. Epub 2021 Mar 17.
Only a few phages have so far been characterized. As several strains are part of probiotic formulations, bacteriophage outbreaks targeting these strains can lead to critical losses within the dairy industry. A new phage was isolated from raw milk obtained from a milking yard from the province of Buenos Aires. The phage genome was sequenced, annotated, and analyzed. Morphology was determined by electron microscopy and the host range was established. phage vB_LcaM_Lbab1 is a member of the family and features a host range including and strains. We further analyzed the baseplate proteins and found putative carbohydrate binding modules that are responsible for host recognition in other phages. A new phage was isolated and characterized. The focus was made on its host recognition mechanism, pointing toward the development of future strategies to avoid deleterious infections in the dairy industry.
到目前为止，只有少数噬菌体得到了表征。由于几种菌株是益生菌制剂的组成部分，针对这些菌株的噬菌体爆发可能会给乳制品行业带来重大损失。从布宜诺斯艾利斯省一个挤奶场获取的生牛奶中分离出一种新的噬菌体。对该噬菌体基因组进行了测序、注释和分析。通过电子显微镜确定了其形态，并确定了宿主范围。噬菌体vB_LcaM_Lbab1是该家族的成员，其宿主范围包括[具体菌株1]和[具体菌株2]菌株。我们进一步分析了基板蛋白，发现了推定的碳水化合物结合模块，这些模块在其他[噬菌体家族名称]噬菌体中负责宿主识别。分离并表征了一种新的噬菌体。重点研究了其宿主识别机制，为未来制定避免乳制品行业有害感染的策略指明了方向。
