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一种来自深海细菌耐压希瓦氏菌WP3的新型丝状噬菌体在低温下被诱导产生。

A novel filamentous phage from the deep-sea bacterium Shewanella piezotolerans WP3 is induced at low temperature.

作者信息

Wang Feng, Wang Fengping, Li Qiang, Xiao Xiang

机构信息

Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen 361005, People's Republic of China.

出版信息

J Bacteriol. 2007 Oct;189(19):7151-3. doi: 10.1128/JB.00569-07. Epub 2007 Jul 27.

Abstract

Active filamentous phage particles were isolated from the deep-sea bacterium Shewanella piezotolerans WP3. A putative single-stranded DNA binding protein of the phage was found to be overexpressed at 4 degrees C compared to its expression at 25 degrees C by two-dimensional polyacrylamide gel electrophoresis. Reverse transcription quantitative PCR further revealed that the key genes of the SW1 phage were significantly induced at low temperature.

摘要

从深海细菌耐压希瓦氏菌WP3中分离出了活性丝状噬菌体颗粒。通过二维聚丙烯酰胺凝胶电泳发现,与在25℃时的表达相比,该噬菌体的一种假定单链DNA结合蛋白在4℃时过表达。逆转录定量PCR进一步显示,SW1噬菌体的关键基因在低温下被显著诱导。

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