Wang Feng, Wang Fengping, Li Qiang, Xiao Xiang
Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen 361005, People's Republic of China.
J Bacteriol. 2007 Oct;189(19):7151-3. doi: 10.1128/JB.00569-07. Epub 2007 Jul 27.
Active filamentous phage particles were isolated from the deep-sea bacterium Shewanella piezotolerans WP3. A putative single-stranded DNA binding protein of the phage was found to be overexpressed at 4 degrees C compared to its expression at 25 degrees C by two-dimensional polyacrylamide gel electrophoresis. Reverse transcription quantitative PCR further revealed that the key genes of the SW1 phage were significantly induced at low temperature.
从深海细菌耐压希瓦氏菌WP3中分离出了活性丝状噬菌体颗粒。通过二维聚丙烯酰胺凝胶电泳发现,与在25℃时的表达相比,该噬菌体的一种假定单链DNA结合蛋白在4℃时过表达。逆转录定量PCR进一步显示,SW1噬菌体的关键基因在低温下被显著诱导。