CAS and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China.
Elife. 2024 Aug 29;13:RP92345. doi: 10.7554/eLife.92345.
Viruses are ubiquitous in nature and play key roles in various ecosystems. Notably, some viruses (e.g. bacteriophage) exhibit alternative life cycles, such as chronic infections without cell lysis. However, the impact of chronic infections and their interactions with the host organisms remains largely unknown. Here, we found for the first time that polysaccharides induced the production of multiple temperate phages infecting two deep-sea strains (WC36 and zth2). Through physiological assays, genomic analysis, and transcriptomics assays, we found these bacteriophages were released via a chronic style without host cell lysis, which might reprogram host polysaccharide metabolism through the potential auxiliary metabolic genes. The findings presented here, together with recent discoveries made on the reprogramming of host energy-generating metabolisms by chronic bacteriophages, shed light on the poorly explored marine virus-host interaction and bring us closer to understanding the potential role of chronic viruses in marine ecosystems.
病毒在自然界中无处不在，在各种生态系统中发挥着关键作用。值得注意的是，一些病毒（例如噬菌体）表现出替代生命周期，例如没有细胞裂解的慢性感染。然而，慢性感染的影响及其与宿主生物的相互作用在很大程度上仍是未知的。在这里，我们首次发现多糖诱导了多种感染两种深海菌株（WC36 和 zth2）的温和噬菌体的产生。通过生理测定、基因组分析和转录组学分析，我们发现这些噬菌体通过慢性方式释放，而不会导致宿主细胞裂解，这可能通过潜在的辅助代谢基因重新编程宿主多糖代谢。这里提出的发现，连同最近关于慢性噬菌体重新编程宿主产生能量代谢的发现一起，揭示了海洋病毒-宿主相互作用这一尚未充分探索的领域，并使我们更接近于理解慢性病毒在海洋生态系统中的潜在作用。
