Jones Malcolm K, Higgins Tess, Stenzel Deborah J, Gobert Geoffrey N
Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4006, Australia.
Exp Parasitol. 2007 Nov;117(3):259-66. doi: 10.1016/j.exppara.2007.06.004. Epub 2007 Jun 21.
One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This technique allows tissues to be dissected for subsequent molecular and protein analysis. The method is reviewed in the light of the acoelomate triploblastic nature of tissue organisation in the parasite.
血吸虫基因组后分析面临的一个困难是，关于寄生虫在其宿主体内表达的许多基因产物的表达位点的数据有限。本文综述了激光显微切割显微镜作为转录组和蛋白质组分析的制备技术的应用潜力。该技术可用于解剖组织，以便进行后续的分子和蛋白质分析。鉴于寄生虫组织结构的无体腔三胚层性质，对该方法进行了综述。
