Yun YeoHeung, Bange Adam, Shanov Vesselin N, Heineman William R, Halsall H Brian, Dong Zhongyun, Jazieh Abdul, Tu Yi, Wong Danny, Pixley Sarah, Behbehani Michael, Schulz Mark J
Department of Mechanical, Industrial, and Nuclear Engineering, Smart Materials Nanotechnology Laboratory, University of Cincinnati, Cincinnati, OH 45221, USA.
J Nanosci Nanotechnol. 2007 Jul;7(7):2293-300. doi: 10.1166/jnn.2007.408.
A carbon nanotube needle biosensor was developed to provide fast, cost effective and highly sensitive electrochemical detection of biomolecules. The sensor was fabricated based on an array of aligned multi-wall carbon nanotubes synthesized by chemical vapor deposition. A bundle of nanotubes in the array was welded onto the tip of a tungsten needle under a microscope. The needle was then encased in glass and a polymer coating leaving only the tip of the needle exposed. Cyclic voltammetry was performed to examine the redox behavior of the nanotube needle. The cyclic voltammetry results showed a steady-state response attributable to radial diffusion with a high steady-state current density. An amperometric sensor was then developed for glucose detection by physically attaching glucose oxidase on the nanotube needle. The amperometric response of these nanotube needles showed a high sensitivity with a low detection limit. It is expected that the nanotube needle can be sharpened to increase the sensitivity to the point where the current is almost too small to measure. The simple manufacturing method should allow commodity level production of highly sensitive electronic biosensors.
一种碳纳米管针状生物传感器被开发出来，用于对生物分子进行快速、经济高效且高度灵敏的电化学检测。该传感器基于通过化学气相沉积法合成的排列整齐的多壁碳纳米管阵列制造而成。在显微镜下，将阵列中的一束纳米管焊接到钨针的尖端。然后将针封装在玻璃和聚合物涂层中，仅使针的尖端暴露在外。进行循环伏安法以研究纳米管针的氧化还原行为。循环伏安法结果显示出归因于径向扩散的稳态响应以及高稳态电流密度。随后通过将葡萄糖氧化酶物理附着在纳米管针上开发了一种用于葡萄糖检测的安培传感器。这些纳米管针的安培响应显示出高灵敏度和低检测限。预计可以将纳米管针磨尖以提高灵敏度，直至电流几乎小到无法测量。这种简单的制造方法应能实现高灵敏度电子生物传感器的商品化生产。
