Wolfson R, Higgins K G, Sears B B
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824.
Mol Biol Evol. 1991 Sep;8(5):709-20. doi: 10.1093/oxfordjournals.molbev.a040680.
Evolutionary relationships of four plastid genomes (plastomes) from different Oenothera species have been assessed by sequence comparisons of two intergenic regions that separate the ribosomal protein genes rpl16, rpl14, and rps8. Sequence changes include base substitutions, the occurrence of a 29-base tandem duplication, and variation in the length of two poly-A stretches. Additions/deletions in chloroplast DNA may not be useful for evolutionary comparisons more distant than these, particularly if the sequences undergo divergence after the initial event, but the length mutations reported here allow a finer resolution of the phylogeny of the closely related Oenothera plastomes than would have been possible if only base substitutions had been considered. Comparisons with the orthogous sequence from tobacco chloroplast DNA indicate the direction of change at most of the sites. The results suggest that plastomes I and II are closely related to each other, as are plastomes III and IV. Replication slippage is proposed as a mechanism to explain the length mutations.
通过对两个基因间隔区进行序列比较，评估了不同月见草物种的四个质体基因组（质体基因组）之间的进化关系，这两个基因间隔区将核糖体蛋白基因rpl16、rpl14和rps8分隔开。序列变化包括碱基替换、一个29碱基串联重复序列的出现以及两个聚腺苷酸序列长度的变化。叶绿体DNA中的插入/缺失对于比这些更远距离的进化比较可能没有用处，特别是如果序列在初始事件后发生分歧，但此处报道的长度突变使得与仅考虑碱基替换相比，能更精细地解析近缘月见草质体基因组的系统发育。与烟草叶绿体DNA的直系同源序列比较表明了大多数位点的变化方向。结果表明质体基因组I和II彼此密切相关，质体基因组III和IV也是如此。提出复制滑动作为解释长度突变的一种机制。
