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与短串联重复序列相关的框内长度突变位于月见草叶绿体DNA的未分配开放阅读框中。

In-frame length mutations associated with short tandem repeats are located in unassigned open reading frames of Oenothera chloroplast DNA.

作者信息

Nimzyk R, Schöndorf T, Hachtel W

机构信息

Botanisches Institut, Universität Bonn, Federal Republic of Germany.

出版信息

Curr Genet. 1993 Mar;23(3):265-70. doi: 10.1007/BF00351505.

DOI:10.1007/BF00351505
PMID:8435856
Abstract

Chloroplast DNAs were compared between two closely related species in the subsection Munzia of the genus Oenothera. A restriction fragment length dimorphism (273 bp) within the large inverted repeats was localized to an unassigned open reading frame that is homologous to ORF 2280 of tobacco chloroplast DNA. This dimorphism is due to different copy numbers of various short tandem repeated sequences, with each repeat unit specifying an in-frame addition or deletion. Other small length mutations were detected within an unassigned reading frame that appears to be homologous to the tobacco ORF 1244, and in the non-coding sequence upstream of that frame. These insertions and/or deletions are all associated with short direct repeats that lie in tandem.

摘要

对月见草属芒齐亚亚组中两个近缘物种的叶绿体DNA进行了比较。在大的反向重复序列中,一个长度为273bp的限制性片段长度多态性定位于一个未定位的开放阅读框,该开放阅读框与烟草叶绿体DNA的ORF 2280同源。这种多态性是由于各种短串联重复序列的拷贝数不同,每个重复单元都指定了一个框内的插入或缺失。在一个似乎与烟草ORF 1244同源的未定位阅读框内以及该框上游的非编码序列中检测到了其他小的长度突变。这些插入和/或缺失都与串联排列的短直接重复序列相关。

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2
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