Chiono Valeria, Pulieri Ettore, Vozzi Giovanni, Ciardelli Gianluca, Ahluwalia Arti, Giusti Paolo
Department of Chemical Engineering, Industrial Chemistry and Materials Science, University of Pisa, Via Diotisalvi 2, Pisa, Italy.
J Mater Sci Mater Med. 2008 Feb;19(2):889-98. doi: 10.1007/s10856-007-3212-5. Epub 2007 Aug 1.
Blends between chitosan (CS) and gelatin (G) with various compositions (CS/G 0/100 20/80, 40/60, 60/40 100/0 w/w) were produced, as candidate materials for biomedical applications. Different amounts of genipin (0.5 wt.% and 2.5 wt.%) were used to crosslink CS/G blends, promoting the formation of amide and tertiary amine bonds between the macromolecules and the crosslinker. The effects of composition and crosslinking on the physico-chemical properties of samples were evaluated by infrared analysis, thermogravimetry, contact angle measurements, dissolution and swelling tests. Mechanical properties of crosslinked samples were also determined through stress-strain and creep tests: samples stiffness increased with increasing the crosslinker amount and the CS content. Blend composition affected mouse fibroblasts adhesion and proliferation on substrates, depending on the crosslinker amount. Finally, crosslinked CS/G blends containing 80 wt.% G were found to support neuroblastoma cells adhesion and proliferation which made them promising candidates for uses in the field of nerve regeneration.
制备了壳聚糖（CS）与明胶（G）的各种组成比例（CS/G 0/100、20/80、40/60、60/40、100/0 w/w）的共混物，作为生物医学应用的候选材料。使用不同量的京尼平（0.5 wt.%和2.5 wt.%）对CS/G共混物进行交联，促进大分子与交联剂之间酰胺键和叔胺键的形成。通过红外分析、热重分析、接触角测量、溶解和溶胀试验评估了组成和交联对样品物理化学性质的影响。还通过应力应变和蠕变试验测定了交联样品的力学性能：样品的刚度随着交联剂用量和CS含量的增加而增加。共混物的组成影响小鼠成纤维细胞在底物上的粘附和增殖，这取决于交联剂的用量。最后，发现含有80 wt.% G的交联CS/G共混物能够支持神经母细胞瘤细胞的粘附和增殖，这使其成为神经再生领域有前景的候选材料。
