Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, 31270.901, Brazil.
J Biomed Nanotechnol. 2010 Apr;6(2):166-75. doi: 10.1166/jbn.2010.1110.
In the present research it is reported the development and characterization of novel polymer blends based on chitosan and poly(vinyl alcohol) (PVA). The hybrid polymeric network was synthesized and modified by chemical crosslinking using genipin for potential use in a variety of biomedical applications. The micro and nanostructures of the blended hydrogels and hybrids were characterized through Fourier Transform Infrared spectroscopy (FTIR) and swelling test analysis. Moreover, cytotoxicity and cell viability were also performed by MTT assay with cell culture. The chemical crosslinking was achieved via the relative intensity of the band at 1650 cm(-1) which is mostly associated with the reaction of genipin carboxymethyl group with the amino group of chitosan forming amides. It was found that by increasing the chitosan content relative to PVA the swelling index of the blend has decreased, reflecting the reduction on the mobility of polymer network and the hydrophilic behavior of the blend. The tested hydrogels have clearly presented adequate cell viability, non-toxicity and suitable properties which can be tailored for potential use in bioengineering applications.
本研究报告了基于壳聚糖和聚乙烯醇（PVA）的新型聚合物共混物的开发和特性研究。通过使用京尼平进行化学交联对杂化聚合物网络进行了合成和修饰，以潜在应用于各种生物医学应用。通过傅里叶变换红外光谱（FTIR）和溶胀试验分析对共混水凝胶和杂化材料的微观和纳米结构进行了表征。此外，还通过细胞培养的 MTT 测定法进行了细胞毒性和细胞活力测试。化学交联是通过在 1650 cm(-1) 处的带的相对强度来实现的，该带主要与京尼平的羧甲基基团与壳聚糖的氨基反应形成酰胺有关。结果发现，随着壳聚糖相对于 PVA 含量的增加，共混物的溶胀指数降低，反映了聚合物网络的流动性降低和共混物的亲水性增强。所测试的水凝胶具有足够的细胞活力、无毒性和合适的特性，可用于生物工程应用。
