Morse Randall H
Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, USA.
J Cell Biochem. 2007 Oct 15;102(3):560-70. doi: 10.1002/jcb.21493.
In eukaryotes, transcription factors, including both gene-specific activators and general transcription factors (GTFs), operate in a chromatin milieu. Here, we review evidence from gene-specific and genome-wide studies indicating that chromatin presents an environment that is typically permissive for activator binding, conditional for pre-initiation complex (PIC) formation, and inhibitory for productive PIC assembly within coding sequences. We also discuss the role of nucleosome dynamics in facilitating access to transcription factors (TFs) in vivo and indicate some of the principal questions raised by recent findings.
在真核生物中，转录因子，包括基因特异性激活因子和通用转录因子（GTFs），在染色质环境中发挥作用。在此，我们综述了来自基因特异性和全基因组研究的证据，这些证据表明染色质呈现出一种环境，该环境通常有利于激活因子结合，对起始前复合物（PIC）形成有条件限制，并且对编码序列内有成效的PIC组装具有抑制作用。我们还讨论了核小体动力学在促进体内转录因子（TFs）可及性方面的作用，并指出了近期研究结果提出的一些主要问题。
