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Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system.

作者信息

Jasin M, Liang F

机构信息

Department of Cell Biology and Genetics, New York, NY.

出版信息

Nucleic Acids Res. 1991 Dec;19(25):7171-5. doi: 10.1093/nar/19.25.7171.

DOI:10.1093/nar/19.25.7171
PMID:1766877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC332558/
Abstract

Mouse embryonic stem (ES) cells were compared to COS1 and CV1 cells for their ability to perform extrachromosomal homologous recombination. RSVCAT plasmid substrates consisting of overlapping chloramphenicol acetyltransferase (CAT) gene fragments were transiently transfected into cells and extracts were assayed for CAT activity. Approximately 10% activity, relative to transfection with a complete CAT gene, was recovered for the recombination substrates in each of the cell lines tested. ES cells, therefore, as other cell lines, are capable of high levels of extrachromosomal recombination.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/ca80a1f02613/nar00183-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/af6791e0737d/nar00183-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/e944b98dd769/nar00183-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/ca80a1f02613/nar00183-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/af6791e0737d/nar00183-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/e944b98dd769/nar00183-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0691/332558/ca80a1f02613/nar00183-0130-a.jpg

相似文献

1
Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system.
Nucleic Acids Res. 1991 Dec;19(25):7171-5. doi: 10.1093/nar/19.25.7171.
2
Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters.胚胎干细胞中通过Cre瞬时表达进行重组的效率：不同启动子的比较
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3
[The characteristics of homologous DNA recombination in somatic cells. II. The characteristics of the extrachromosomal recombination of plasmid DNA in cultured cells differing in their tumor potential].[体细胞中同源DNA重组的特征。II. 具有不同肿瘤潜能的培养细胞中质粒DNA的染色体外重组特征]
Tsitologiia. 1993;35(4):107-14.
4
Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells.位点特异性核酸内切酶的表达刺激哺乳动物细胞中的同源重组。
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Transcription from plasmid expression vectors is increased up to 14-fold when plasmids are transfected as concatemers.当质粒以串联体形式转染时，来自质粒表达载体的转录增加高达14倍。
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[The characteristics of homologous DNA recombination in somatic cells. I. The construction of a DNA plasmid containing deletion variants of the aminoglycoside phosphoribosyltransferase gene].[体细胞中同源DNA重组的特征。I. 含有氨基糖苷磷酸核糖基转移酶基因缺失变体的DNA质粒的构建]
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Prolonged transgene expression in rodent lung cells.
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Embryonic stem (ES) cells lacking functional c-jun: consequences for growth and differentiation, AP-1 activity and tumorigenicity.缺乏功能性c-jun的胚胎干细胞：对生长、分化、AP-1活性和致瘤性的影响
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A stable cellular marker for the analysis of mouse chimeras: the bacterial chloramphenicol acetyltransferase gene driven by the human elongation factor 1 alpha promoter.一种用于分析小鼠嵌合体的稳定细胞标志物：由人延伸因子1α启动子驱动的细菌氯霉素乙酰转移酶基因。
Differentiation. 1991 Dec;48(3):183-9. doi: 10.1111/j.1432-0436.1991.tb00256.x.

引用本文的文献

1
Mouse embryonic stem cells, but not somatic cells, predominantly use homologous recombination to repair double-strand DNA breaks.小鼠胚胎干细胞，但不是体细胞，主要利用同源重组修复双链 DNA 断裂。
Stem Cells Dev. 2010 Nov;19(11):1699-711. doi: 10.1089/scd.2010.0058. Epub 2010 Aug 5.
2
Chromosomal double-strand break repair in Ku80-deficient cells.Ku80 缺陷细胞中的染色体双链断裂修复
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8929-33. doi: 10.1073/pnas.93.17.8929.
3
Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells.

本文引用的文献

1
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
2
Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.合成寡核苷酸插入突变体在哺乳动物细胞同源重组研究中的新应用。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4827-31. doi: 10.1073/pnas.80.15.4827.
3
The double-strand-break repair model for recombination.用于重组的双链断裂修复模型。
靶位点的双链断裂可刺激胚胎干细胞中的基因靶向作用。
Nucleic Acids Res. 1995 Dec 25;23(24):5012-9. doi: 10.1093/nar/23.24.5012.
4
Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells.位点特异性核酸内切酶的表达刺激哺乳动物细胞中的同源重组。
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6064-8. doi: 10.1073/pnas.91.13.6064.
5
Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease.通过表达一种稀有切割的核酸内切酶将双链断裂引入小鼠细胞基因组。
Mol Cell Biol. 1994 Dec;14(12):8096-106. doi: 10.1128/mcb.14.12.8096-8106.1994.
Cell. 1983 May;33(1):25-35. doi: 10.1016/0092-8674(83)90331-8.
4
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.DNA转入小鼠L细胞过程中的同源重组模型：DNA末端在重组过程中的作用。
Mol Cell Biol. 1984 Jun;4(6):1020-34. doi: 10.1128/mcb.4.6.1020-1034.1984.
5
Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.通过对输入DNA进行处理，可以增强哺乳动物细胞中质粒间的同源重组。
Proc Natl Acad Sci U S A. 1984 May;81(10):3153-7. doi: 10.1073/pnas.81.10.3153.
6
Recombination during gene transfer into mouse cells can restore the function of deleted genes.在将基因导入小鼠细胞的过程中发生的重组可以恢复缺失基因的功能。
Science. 1983 Jan 14;219(4581):174-6. doi: 10.1126/science.6294829.
7
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.劳氏肉瘤病毒长末端重复序列通过DNA介导转染导入多种真核细胞时是一个强启动子。
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81. doi: 10.1073/pnas.79.22.6777.
8
Yeast transformation: a model system for the study of recombination.酵母转化：用于重组研究的模型系统。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8. doi: 10.1073/pnas.78.10.6354.
9
SV40-transformed simian cells support the replication of early SV40 mutants.猴空泡病毒 40（SV40）转化的猿猴细胞支持早期 SV40 突变体的复制。
Cell. 1981 Jan;23(1):175-82. doi: 10.1016/0092-8674(81)90282-8.
10
Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.哺乳动物细胞中的同源重组介导了由两个重叠基因片段形成一个功能基因的过程。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2002-6. doi: 10.1073/pnas.80.7.2002.