Bianchi D W, Stewart J E, Garber M F, Lucotte G, Flint A F
Division of Genetics, Children's Hospital, Boston, MA 02115.
Prenat Diagn. 1991 Aug;11(8):523-8. doi: 10.1002/pd.1970110807.
Maternal venous blood samples, obtained from six pregnant women, were used as a source of fetal nucleated erythrocytes (NRBC). Fetal cell enrichment was potentiated by flow sorting with the monoclonal antibodies TfR, Leu-4, and Leu-M3. Single copy Y chromosomal DNA sequences were detected in samples obtained from two women at 11 and 12 weeks' gestation. Y DNA sequences were absent in a subsequent sample from one of these women at 19 weeks and in two other women at 16 and 20 weeks. All four women delivered males. Y DNA sequences were not detected in two women who delivered females. By combining these results with prior data on the detection of Y chromosomal DNA sequences in maternal blood from male-bearing pregnancies, a relationship between gestational age and feto-maternal transfer of NRBC is suggested.
从六名孕妇采集的母静脉血样本被用作胎儿有核红细胞（NRBC）的来源。通过使用单克隆抗体TfR、Leu-4和Leu-M3进行流式分选增强了胎儿细胞富集。在妊娠11周和12周时从两名女性获得的样本中检测到单拷贝Y染色体DNA序列。其中一名女性在19周时的后续样本以及另外两名女性在16周和20周时的样本中未检测到Y DNA序列。这四名女性均分娩出男性。在两名分娩出女性的女性中未检测到Y DNA序列。通过将这些结果与先前关于在怀有男性胎儿的孕妇血液中检测Y染色体DNA序列的数据相结合，提示了胎龄与NRBC的母婴转移之间的关系。
