Wachtel S, Elias S, Price J, Wachtel G, Phillips O, Shulman L, Meyers C, Simpson J L, Dockter M
Department of Obstetrics and Gynecology, University of Tennessee, Memphis 38163.
Hum Reprod. 1991 Nov;6(10):1466-9. doi: 10.1093/oxfordjournals.humrep.a137290.
During pregnancy, nucleated fetal erythrocytes enter the maternal circulation and can be isolated efficiently from the maternal cells by multiparameter flow cytometry. Male DNA, implying presence of a male fetus, can be identified in flow-sorted maternal blood by polymerase chain reaction with oligonucleotide primers flanking single-copy Y-specific DNA sequences. Among flow-sorted samples, we correctly identified fetal sex in 17/18 (94%) pregnancies of 10-21 weeks gestation. Maternal blood thus provides a potential opportunity for prenatal diagnosis that could preclude the need for invasive procedures in current use.
在怀孕期间，有核胎儿红细胞进入母体循环，并且可以通过多参数流式细胞术从母体细胞中高效分离出来。通过使用侧翼为单拷贝Y特异性DNA序列的寡核苷酸引物进行聚合酶链反应，可在流式分选的母血中鉴定出意味着存在男性胎儿的男性DNA。在流式分选的样本中，我们在10 - 21周妊娠的18例妊娠中的17例（94%）中正确鉴定了胎儿性别。因此，母血为产前诊断提供了一个潜在的机会，这可能排除当前使用的侵入性程序的必要性。
