Lucchi Géraldine, Hendra Jean-Baptiste, Pecqueur Delphine, Ducoroy Patrick
Plate-forme Protéomique IFR-Santé-STIC, 8, boulevard de Lattre de Tassigny, 21000 Dijon, France.
Med Sci (Paris). 2007 Mar;23 Spec No 1:19-22. doi: 10.1051/medsci/2007231s19.
Proteins identified in biological fluids of cancer patients could be helpful for both diagnosis and prognosis. However, clinical proteomics based on analysis of protein profiles in biological fluids has demonstrated various flaws, most of them related to the difficulties met in reproducibility. These difficulties could be partly overcome by accurate standardisation of pre-analytical and analytical steps of these studies. The size of the patient cohort is one of the parameters that determine the powerfulness of the study. Recruitment of a cohort with a sufficient size often implies multicentric studies in which analysis of the reproducibility between centres and standardisation of pre-analytical and analytical steps are essential. Such a standardisation requires the use of calibrated samples as common references.
在癌症患者生物体液中鉴定出的蛋白质可能对诊断和预后均有帮助。然而，基于生物体液中蛋白质谱分析的临床蛋白质组学已显示出各种缺陷，其中大多数与再现性方面遇到的困难有关。通过对这些研究的分析前和分析步骤进行准确标准化，这些困难可以得到部分克服。患者队列的规模是决定研究效能的参数之一。招募足够规模的队列通常意味着要进行多中心研究，其中各中心之间再现性的分析以及分析前和分析步骤的标准化至关重要。这种标准化需要使用经过校准的样本作为通用参考。
