通过体内电穿孔增强对疟疾DNA疫苗的抗体和细胞免疫反应。
Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation.
作者信息
Dobaño Carlota, Widera Georg, Rabussay Dietmar, Doolan Denise L
机构信息
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, United States.
出版信息
Vaccine. 2007 Sep 4;25(36):6635-45. doi: 10.1016/j.vaccine.2007.06.036. Epub 2007 Jul 10.
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 microg of DNA via EP was equivalent to 50 microg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
我们评估了体内电穿孔（EP）对增强免疫反应的有效性，该免疫反应由编码约氏疟原虫红细胞前期抗原PyCSP和PyHEP17的DNA质粒诱导产生，这些质粒通过肌肉注射和皮内注射给予小鼠。电穿孔分别使针对PyCSP和PyHEP17的抗体反应增强了16倍和2倍。通过电穿孔用5微克DNA进行免疫相当于通过传统针头用50微克DNA进行免疫，从而将产生给定效果所需的剂量减少了10倍。此外，γ干扰素反应增加了约2倍。数据证明了电穿孔增强针对感染因子的DNA疫苗免疫反应的潜力。
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