Song Jihwan, Lee Soon-Tae, Kang Wonsuk, Park Jung-Eun, Chu Kon, Lee Song-ee, Hwang Taesun, Chung Hyungmin, Kim Manho
CHA Stem Cell Institute, Pochon CHA University College of Medicine, and Department of Neurology, Clinical Research Institute, Seoul National University Hospital, South Korea.
Neurosci Lett. 2007 Aug 9;423(1):58-61. doi: 10.1016/j.neulet.2007.05.066. Epub 2007 Jul 14.
To test the efficacy of human embryonic stem cell (hESC)-derived neural precursors in an experimental model of Huntington's disease (HD), we differentiated hESC into nestin-positive neural precursors by co-culturing with PA6 stromal cells, and subsequently transplanted them into the striatum of quinolinic acid (QA)-induced HD model. The transplanted animals exhibited a behavioral recovery in the apomorphine-induced rotation test for 3 weeks after transplantation. The transplanted hESC-derived neural precursors were found in both cortex and striatum. They also exhibited some evidence of neuronal differentiation. At the time of examination, no tumor was detected. These results strongly suggest that hESC-derived neural precursors can lead to a behavioral recovery, as well as neuronal differentiation, in the pre-clinical model of HD.
为了在亨廷顿舞蹈症（HD）实验模型中测试人胚胎干细胞（hESC）来源的神经前体细胞的功效，我们通过与PA6基质细胞共培养，将hESC分化为巢蛋白阳性神经前体细胞，随后将它们移植到喹啉酸（QA）诱导的HD模型的纹状体中。移植后的动物在移植后3周的阿扑吗啡诱导旋转试验中表现出行为恢复。在皮层和纹状体中均发现了移植的hESC来源的神经前体细胞。它们还表现出一些神经元分化的迹象。在检查时，未检测到肿瘤。这些结果有力地表明，在HD临床前模型中，hESC来源的神经前体细胞可导致行为恢复以及神经元分化。
