鸟分枝杆菌副结核亚种I型、II型和III型分离株中gyrA和gyrB基因的多态性
Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates.
作者信息
Castellanos E, Aranaz A, Romero B, de Juan L, Alvarez J, Bezos J, Rodríguez S, Stevenson K, Mateos A, Domínguez L
机构信息
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.
出版信息
J Clin Microbiol. 2007 Oct;45(10):3439-42. doi: 10.1128/JCM.01411-07. Epub 2007 Aug 1.
Abstract
The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.
摘要
对一组来自I型、II型和III型副结核分枝杆菌鸟分枝杆菌亚种分离株的gyrA和gyrB基因进行分析，检测到了型特异性单核苷酸多态性。基于这些结果，我们开发了一种PCR和限制性酶切分析方法来区分I型和III型分离株。当细菌生长量不足以进行脉冲场凝胶电泳和IS900限制性片段长度多态性分析时，应用该技术将是鉴定这些菌株的唯一策略。
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