Suzuki Tsutomu, Ikeuchi Yoshiho, Noma Akiko, Suzuki Takeo, Sakaguchi Yuriko
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Methods Enzymol. 2007;425:211-29. doi: 10.1016/S0076-6879(07)25009-8.
Posttranscriptional modifications are characteristic structural features of RNA molecules. To study the functional roles played by RNA modifications, it is necessary to identify the genes and enzymes that are responsible for their biosynthesis. Many uncharacterized genes for RNA modifications still remain buried in the genomes of model organisms. We describe here a systematic genomewide screening method that uses a reverse genetic approach combined with mass spectrometry, which we have named "ribonucleome analysis," to identify uncharacterized genes that are involved in generating RNA modifications.
转录后修饰是RNA分子的典型结构特征。为了研究RNA修饰所发挥的功能作用，有必要鉴定负责其生物合成的基因和酶。许多未被表征的RNA修饰基因仍隐藏在模式生物的基因组中。我们在此描述一种系统的全基因组筛选方法，该方法采用反向遗传学方法并结合质谱分析，我们将其命名为“核糖核酸组分析”，以鉴定参与产生RNA修饰的未被表征的基因。
