Gao Xiao-xia, Yan Han-jing, Liang Cong-qing, Chen Xiao-ying
Guangdong University of Pharmacy, Guangzhou 510006, China.
Zhong Yao Cai. 2007 Apr;30(4):407-9.
To establish thin-layer chromatography (TLC) fingerprint of Radix Polygoni Multiflori collected from different areas.
Quantitative analysis was carried out using TLC scanning method, two mixed solvents of petroleum ether (60-90 degrees C)-ethylacetate-formic acid (15:5:1) and chloroform-methanol-water [6.5: 2.25: 0.42, pH = 4.0 (HAc-NaAc buffer)] were employed as the mobile phase.
The TLC chromatograms were constituted 14 characteristic common peaks. The plots based on the height of common peaks can be conveniently used to evaluate the quality of different area samples.
The TLC fingerprints can be used to classify and identify Radix Polygoni Multiflori rapidly and effectively.
建立不同产地何首乌的薄层色谱（TLC）指纹图谱。
采用薄层色谱扫描法进行定量分析，以石油醚（60 - 90℃）-乙酸乙酯-甲酸（15:5:1）和三氯甲烷-甲醇-水[6.5:2.25:0.42，pH = 4.0（醋酸-醋酸钠缓冲液）]两种混合溶剂为流动相。
薄层色谱图中共有14个特征共有峰。基于共有峰峰高绘制的图谱可方便地用于评价不同产地样品的质量。
该薄层色谱指纹图谱可快速、有效地用于何首乌的分类和鉴别。
