Brown Ashley N, Kent Kim A, Bennett Corey J, Bernard Kristen A
Wadsworth Center, New York State Department of Health, P.O. Box 509, Albany, NY 12201-0509, USA.
Virology. 2007 Nov 25;368(2):422-30. doi: 10.1016/j.virol.2007.06.033. Epub 2007 Aug 6.
West Nile virus (WNV) is a mosquito-borne flavivirus that infects the central nervous system of humans and other animals. In this study, we found that C3H/HeN (C3H) mice exhibited a higher morbidity and mortality than C57BL/6 (B6) mice. We compared tissue tropism, viral replication and kinetics for C3H and B6 mice during acute viral infection. WNV was detected in multiple tissues, including novel sites such as the skin, duodenum and pancreas, but the tropism was identical for the two strains. Additionally, viral load and kinetics of spread did not differ substantially between strains. Neuroinvasion occurred in both strains by day 3 post-inoculation with early detection in the olfactory bulbs and spinal cord, suggesting that WNV neuroinvades at specific sites. Furthermore, neuroinvasion and viral load in the CNS did not predict disease outcome. Our data suggest that the disparities in morbidity and mortality between C3H and B6 mice are not due to differences in tropism, viral load or kinetics during acute WNV infection.
西尼罗河病毒（WNV）是一种由蚊子传播的黄病毒，可感染人类和其他动物的中枢神经系统。在本研究中，我们发现C3H/HeN（C3H）小鼠比C57BL/6（B6）小鼠表现出更高的发病率和死亡率。我们比较了急性病毒感染期间C3H和B6小鼠的组织嗜性、病毒复制和动力学。在包括皮肤、十二指肠和胰腺等新部位在内的多个组织中检测到了WNV，但两种品系的嗜性相同。此外，品系之间的病毒载量和传播动力学没有显著差异。接种后第3天，两种品系均发生神经侵袭，在嗅球和脊髓中早期检测到，这表明WNV在特定部位发生神经侵袭。此外，CNS中的神经侵袭和病毒载量并不能预测疾病结果。我们的数据表明，C3H和B6小鼠在发病率和死亡率上的差异并非由于急性WNV感染期间嗜性、病毒载量或动力学的差异。
