Sopp Paul, Werling Dirk, Baldwin Cynthia
Institute for Animal Health, Compton RG20 7NN, UK.
Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):106-14. doi: 10.1016/j.vetimm.2007.06.014. Epub 2007 Jun 20.
A panel of 377 commercially available mAbs were submitted to the animal homologue section of the 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) for cross-reactivity studies on different animal species. In this study we describe the results of testing the mAbs on cattle cells by flow cytometry and Western blot. Eight commercial suppliers participated, providing mAbs to a total of 144 CD antigens plus controls. Fifty-two mAbs were identified as potentially staining cattle cells in the first round screen. In the second phase, 38 mAbs were confirmed as staining cattle cells. This included some that may recognise polymorphic determinants and others with atypical distribution patterns compared to humans. mAb to human CD9, CD11a, CD14, CD18, CD21, CD23, CD29, CD44, CD45R, CD47, CD49d and CD172a cross-reacted with bovine cells and mAb to CD22, CD88, CD119 and CD163 stained CD antigens that have not previously been identified in cattle.
一组377种市售单克隆抗体被提交至第八届人类白细胞分化抗原国际研讨会（HLDA8，澳大利亚阿德莱德）的动物同源物部分，用于在不同动物物种上进行交叉反应性研究。在本研究中，我们描述了通过流式细胞术和蛋白质印迹法在牛细胞上测试这些单克隆抗体的结果。八家商业供应商参与其中，提供了针对总共144种CD抗原及对照的单克隆抗体。在第一轮筛选中，有52种单克隆抗体被鉴定为可能对牛细胞染色。在第二阶段，38种单克隆抗体被确认为能对牛细胞染色。这包括一些可能识别多态性决定簇的抗体，以及其他与人类相比具有非典型分布模式的抗体。针对人类CD9、CD11a、CD14、CD18、CD21、CD23、CD29、CD44、CD45R、CD47、CD49d和CD172a的单克隆抗体与牛细胞发生交叉反应，而针对CD22、CD88、CD119和CD163的单克隆抗体则对牛中以前未鉴定出的CD抗原进行了染色。
