牛和其他物种间脂肪干细胞和前体细胞中的标记物和干细胞特性的保存。
Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species.
机构信息
Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Department of Clinical Regenerative Medicine, Fujita Health University, Toyoake 470-1192, Japan.
出版信息
Int J Mol Sci. 2023 Jul 25;24(15):11908. doi: 10.3390/ijms241511908.
Adipose stem and progenitor cells (ASPCs) have been isolated from humans and animals for use in regenerative medicine and therapy. However, knowledge of ASPCs in other species is limited. Particularly, ASPCs in livestock are expected to enhance the fat content and meat composition. In this study, we isolated bovine ASPCs using cell surface markers. Specifically, we focused on ASPC markers in humans and experimental animals, namely CD26, CD146, and CD54. Stromal vascular fraction cells from bovine fat were separated using flow cytometry before primary culture. We evaluated the self-renewal and adipogenic potential of each fraction. We identified four cell populations: CD26-CD146+CD54+, CD26-CD146+CD54-, CD26-CD146-, and CD26+CD146-. Among them, the CD26-CD146+ fraction, particularly CD54+, demonstrated the properties of preadipocytes (PreAs), characterized by slow proliferation and a high adipogenic capacity. In conclusion, we could collect and characterize possible PreAs as CD26-CD146+CD54+ or CD26-CD146+CD54-, which are expected for in vitro bovine adipogenic assays in the future.
脂肪干细胞和祖细胞 (ASPCs) 已从人类和动物中分离出来，用于再生医学和治疗。然而，其他物种的 ASPC 知识有限。特别是，家畜中的 ASPC 有望提高脂肪含量和肉组成。在这项研究中，我们使用细胞表面标志物从牛中分离出 ASPC。具体来说，我们专注于人类和实验动物中的 ASPC 标志物，即 CD26、CD146 和 CD54。使用流式细胞术分离牛脂肪的基质血管部分细胞，然后进行原代培养。我们评估了每个部分的自我更新和脂肪生成潜力。我们鉴定了四个细胞群体：CD26-CD146+CD54+、CD26-CD146+CD54-、CD26-CD146-和 CD26+CD146-。其中，CD26-CD146+部分，特别是 CD54+，表现出前脂肪细胞（PreAs）的特性，其特征为增殖缓慢和高脂肪生成能力。总之，我们可以收集和表征可能的 PreAs 为 CD26-CD146+CD54+或 CD26-CD146+CD54-，这有望用于未来的体外牛脂肪生成测定。
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