Xiao J H, Zhang J H, Zhang Y Y, Wang T T, Chen R G, Li H X, Ye Z B
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, People's Republic of China.
DNA Seq. 2007 Dec;18(6):474-9. doi: 10.1080/10425170701462183.
GA 2-oxidases, a key enzyme involves GA biosynthesis, catalyze the degradation of active C(19)-Gibberellins (GAs) through 2-hydroxylation yields inactive GA product. Searching public tomato database, the putative GA2ox2 sequences were assembled. We isolated a full-length GA2ox2 cDNA with primers designed from the assembled sequence. This gene was designed as SlGA2ox2 (GenBank accession No. EF017805). The full-length GA2ox2 gene contained a complete open reading frame (ORF) of 1203 bp, which encoded 322 amino acid residues. Amino acid sequence homology analysis of SlGA2ox2 showed an 88% identity with NtGA2ox2 in tobacco. And alignments of SlGA2ox2 with other known GA2ox from Arabidopsis, Pea, Adzuki Bean, Winter Squash etc indicate low similarity of 47-70%. Semi-quantitative RT-PCR analysis showed a specific expression profile of SlGA2ox2 in different tissues, which mainly expressed in flowers and traces were detected in roots, stems, leaves and immature fruits.
GA 2-氧化酶是赤霉素生物合成中的关键酶，它通过2-羟基化作用催化活性C(19)-赤霉素（GAs）的降解，产生无活性的GA产物。通过搜索公共番茄数据库，拼接出了假定的GA2ox2序列。我们根据拼接序列设计引物，分离出了一个全长GA2ox2 cDNA。该基因被命名为SlGA2ox2（GenBank登录号：EF017805）。GA2ox2全长基因包含一个1203 bp的完整开放阅读框（ORF），编码322个氨基酸残基。SlGA2ox2的氨基酸序列同源性分析表明，它与烟草中的NtGA2ox2有88%的同一性。SlGA2ox2与拟南芥、豌豆、小豆、西葫芦等其他已知GA2ox的比对显示，相似性较低，为47%-70%。半定量RT-PCR分析显示，SlGA2ox2在不同组织中有特定的表达模式，主要在花中表达，在根、茎、叶和未成熟果实中检测到微量表达。
