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[从猪和牛血清中获取β-球蛋白组分及其免疫活性研究]

[Obtaining the beta-globulin fractions from swine and cattle sera and a study of their immunological activity].

作者信息

Evtimov M, Iotov M

出版信息

Vet Med Nauki. 1976;13(1):37-47.

PMID:176771
Abstract

The rivanol precipitation was used to obtain beta-globulin fractions from specific swine sera against edema disease, paratyph and Aujeszky's disease as well as from normal ovine and swine sera. Agar electrophoresis revealed that the preparations produced contained beta-globulin (86 per cent), gamma-globulin (5 per cent), and alpha2-globulin (9 per cent). The beta-globulin preparations were studied for the presence of antibodies against E. coli, Salmonellae, staphylococci, myxovirus parainfluenza-3, adenoviruses, and the virus of Aujeszky's disease. The beta-globulin fraction of the specific serum against edema disease was shown to contain OK and O agglutinizing antibodies against E. coli, having a titer of up to 1:3200, and the gamma-globulin titers of up to 1:12800. The beta-globulin fraction of the specific serum against typhoid contained OH and O agglutinizing antibodies against Salmonella cholerae suis with a titer of up to 1:2560. The agglutinizing antibodies against staphylococci were twice as much in the beta-globulin fraction as compared with those in the gamma-globulin fraction. The antibodies against Aujeszky's disease and the adenoviruses in the beta-globulin fractions were in negligible amounts, and the titer of the antibodies against myxovirus parainfluenza-3 ranged from 1:128 to 1:512.

摘要

采用利凡诺沉淀法从抗水肿病、副伤寒和奥耶斯基氏病的猪特异性血清以及正常绵羊和猪血清中获取β-球蛋白组分。琼脂电泳显示,所制备的产物含有β-球蛋白(86%)、γ-球蛋白(5%)和α2-球蛋白(9%)。对β-球蛋白制剂进行了研究,检测其中针对大肠杆菌、沙门氏菌、葡萄球菌、副流感病毒3型、腺病毒和奥耶斯基氏病病毒的抗体。结果表明,抗水肿病特异性血清的β-球蛋白组分含有针对大肠杆菌的OK和O凝集抗体,效价高达1:3200,γ-球蛋白效价高达1:12800。抗伤寒特异性血清的β-球蛋白组分含有针对猪霍乱沙门氏菌的OH和O凝集抗体,效价高达1:2560。β-球蛋白组分中针对葡萄球菌的凝集抗体量是γ-球蛋白组分中的两倍。β-球蛋白组分中针对奥耶斯基氏病和腺病毒的抗体量可忽略不计,针对副流感病毒3型的抗体效价在1:128至1:512之间。

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