Heijna M C R, van Enckevort W J P, Vlieg E
IMM, Solid State Chemistry, Radboud University Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands.
Phys Rev E Stat Nonlin Soft Matter Phys. 2007 Jul;76(1 Pt 1):011604. doi: 10.1103/PhysRevE.76.011604. Epub 2007 Jul 20.
In the phase diagram of the protein hen egg-white lysozyme, a region is present in which the lysozyme solution demixes and forms two liquid phases. In situ observations by optical microscopy show that the dense liquid droplets dissolve when crystals grow in this system. During this process the demixed liquid region retracts from the crystal surface. The spatial distribution of the dense phase droplets present special boundary conditions for Fick's second law for diffusion. In combination with the cylindrical symmetry provided by the kinetically roughened crystals, this system allows for a full numerical analysis. Using experimental data for setting the boundary conditions, a quasi-steady-state solution for the time-dependent concentration profile was shown to be valid. Comparison of kinetically rough growth in a phase separated system and in a nonseparated system shows that the growth kinetics for a three-phase system differs from a two-phase system, in that crystals grow more slowly but the duration of growth is prolonged.
在蛋白质鸡蛋清溶菌酶的相图中，存在一个区域，在该区域溶菌酶溶液会发生相分离并形成两个液相。光学显微镜的原位观察表明，当该系统中晶体生长时，致密液滴会溶解。在此过程中，相分离的液相区域会从晶体表面缩回。致密相液滴的空间分布为菲克第二扩散定律提供了特殊的边界条件。结合动力学粗糙化晶体所提供的柱对称性，该系统允许进行完整的数值分析。利用实验数据设定边界条件，结果表明与时间相关的浓度分布的准稳态解是有效的。相分离系统和非相分离系统中动力学粗糙生长的比较表明，三相系统的生长动力学与两相系统不同，即晶体生长较慢，但生长持续时间延长。
