Sevin Emeric W, Barloy-Hubler Frédérique
CNRS UMR6061 Génétique et Développement, Université de Rennes 1, IFR 140, Av, du Prof, Léon Bernard, CS 34317, 35043 Rennes, France.
Genome Biol. 2007;8(8):R155. doi: 10.1186/gb-2007-8-8-r155.
Toxin/antitoxin (TA) systems, viewed as essential regulators of growth arrest and programmed cell death, are widespread among prokaryotes, but remain sparsely annotated. We present RASTA-Bacteria, an automated method allowing quick and reliable identification of TA loci in sequenced prokaryotic genomes, whether they are annotated open reading frames or not. The tool successfully confirmed all reported TA systems, and spotted new putative loci upon screening of sequenced genomes. RASTA-Bacteria is publicly available at http://genoweb.univ-rennes1.fr/duals/RASTA-Bacteria.
毒素/抗毒素（TA）系统被视为生长停滞和程序性细胞死亡的重要调节因子，在原核生物中广泛存在，但注释仍然很少。我们提出了RASTA-Bacteria，这是一种自动化方法，可快速可靠地识别已测序原核基因组中的TA基因座，无论它们是否为注释的开放阅读框。该工具成功确认了所有已报道的TA系统，并在筛选已测序基因组时发现了新的假定基因座。RASTA-Bacteria可在http://genoweb.univ-rennes1.fr/duals/RASTA-Bacteria上公开获取。
