Johnsson Richard, Träff Gustav, Sundén Martin, Ellervik Ulf
Organic Chemistry, Lund University, SE-221 00, Lund, Sweden.
J Chromatogr A. 2007 Sep 14;1164(1-2):298-305. doi: 10.1016/j.chroma.2007.07.029. Epub 2007 Jul 21.
Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modern image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds.
使用染色试剂的薄层色谱法（TLC）是分析无发色团有机化合物的一种优越方法。它快速、通用，有时是唯一可行的方法。我们研究了将使用染色试剂的定量TLC与现代图像分析软件相结合的方法。我们的结果表明，有可能获得适用于高通量筛选或物理有机研究的可靠测量结果。文中阐述了该过程不同部分的检测范围和误差。我们表明，误差主要源于染色过程，通过测量化合物的比例可以减小误差。
