We propose a technique for the automated detection of malignant masses in screening mammography. The technique is based on the presence of concentric layers surrounding a focal area with suspicious morphological characteristics and low relative incidence in the breast region. Mammographic locations with high concentration of concentric layers with progressively lower average intensity are considered suspicious deviations from normal parenchyma. The multiple concentric layers (MCLs) technique was trained and tested using the craniocaudal views of 270 mammographic cases with biopsy proven malignant masses from the digital database of screening mammography. One-half of the available cases were used for optimizing the parameters of the detection algorithm. The remaining cases were used for testing. During testing, malignant masses were detected with 92%, 88%, and 81% sensitivity at 5.4, 2.4, and 0.6 false positive marks per image. Testing on 82 normal screening mammograms showed a false positive rate of 5.0, 1.7, and 0.2 marks per image at the previously reported operating points. Furthermore, additional evaluation on 135 benign cases produced a significantly lower detection rate for benign masses (61.6%, 58.3%, and 43.7% at 5.1, 2.8, and 1.2 false positives per image, respectively). Overall, MCL is a promising computer-assisted detection strategy for screening mammograms to identify malignant masses while maintaining the detection rate of benign masses considerably lower.