Matheson Jodi S, Paul-Murphy Joanne, O'Brien Robert T, Steinberg Howard
University of Wisconsin-Madison, School of Veterinary Medicine, 2015 Linden Drive, Madison, Wisconsin 53706, USA.
J Zoo Wildl Med. 2007 Jun;38(2):222-30. doi: 10.1638/1042-7260(2007)038[0222:QUMRIA]2.0.CO;2.
Iron overload was induced by iron dextran i.v. in clinically healthy adult pigeons, Columbia livia, (n = 8). Hemosiderosis was induced in all treated birds. Two control pigeons received no iron injections. Pigeons did not show clinical signs of iron overload during the 6-wk study. Ultrasound examination of the liver in the pigeons receiving iron dextran was performed on days 0, 13, 28, and 42. No ultrasound images were collected on the control pigeons. Magnetic resonance imaging was performed on days 0, 13, 28, and 42 on all study pigeons and imaging sequences were collected in three different imaging formats: T1, T2, and gradient-recalled echo (GRE). Surgical liver biopsies were performed on pigeons receiving iron dextran on days 2, 16, and 45 (at necropsy). A single liver sample was collected at necropsy from the control birds. Histologic examination, quantitative image analysis, and tissue iron analysis by thin-layer chromatography were performed on each liver sample and compared to the imaging studies. Although hemosiderosis was confirmed histologically in each experimental pigeon, no significant change in pixel intensity of the ultrasound images was seen at any point in the study. Signal intensity, in all magnetic resonance imaging formats, significantly decreased in a linear fashion as the accumulation of iron increased.
通过静脉注射葡聚糖铁在临床健康的成年家鸽（哥伦比亚家鸽，n = 8）中诱导铁过载。所有接受治疗的鸟类均诱发了含铁血黄素沉着症。两只对照鸽未接受铁注射。在为期6周的研究中，鸽子未表现出铁过载的临床症状。在第0、13、28和42天对接受葡聚糖铁的鸽子进行肝脏超声检查。对照鸽未采集超声图像。在第0、13、28和42天对所有研究鸽进行磁共振成像，并以三种不同的成像格式采集成像序列：T1、T2和梯度回波（GRE）。在第2、16和45天（尸检时）对接受葡聚糖铁的鸽子进行手术肝活检。在尸检时从对照鸟采集单个肝脏样本。对每个肝脏样本进行组织学检查、定量图像分析和薄层色谱法组织铁分析，并与成像研究进行比较。尽管在组织学上证实每只实验鸽均有含铁血黄素沉着症，但在研究的任何时间点，超声图像的像素强度均未观察到显著变化。随着铁积累的增加，在所有磁共振成像格式中，信号强度均呈线性显著下降。
