Rodriguez Barbon Alberto, Bentley Charlotte, Drake Gabby J, Veiga Miguel Mendes, Chantrey Julian, Rocchigiani Guido
Chester Zoo, Chester, UK.
Scotland's Rural College, Midlothian, UK.
Vet Pathol. 2025 May;62(3):360-363. doi: 10.1177/03009858241309395. Epub 2024 Dec 30.
Hepatic hemosiderosis is a common nutritional disease affecting numerous bird species. The aim of this study was to validate the use of pinch hepatic biopsies to quantify the hepatic iron in Asian glossy starlings (AGS) and compare clinical measures usable for antemortem disease monitoring. Following euthanasia, serum, liver pinch biopsies, and whole liver sections were sampled from 21 AGS. Liver biopsies and sections were stained with Perl's Prussian blue, and the area containing iron pigment was calculated by image analysis. The mean percent iron-stained area per whole hepatic section was 62.52% ± 21.81%, while the mean percent iron-stained area in biopsies was 71.47% ± 19.77%. Positive correlations were found between hepatic iron concentration and percentage iron in whole sections ( = 0.92; < .0001), percentage iron in biopsies ( = 0.91; < .0001), and other clinico-pathological features. Hemosiderin in hepatic biopsies was significantly higher than in whole hepatic section assessed by image analysis ( < .0001).
肝铁沉积症是一种影响众多鸟类的常见营养性疾病。本研究的目的是验证采用肝穿刺活检来量化亚洲辉椋鸟（AGS）肝脏铁含量的方法，并比较可用于生前疾病监测的临床指标。对21只亚洲辉椋鸟实施安乐死后，采集其血清、肝脏穿刺活检样本和全肝切片。肝脏活检样本和切片用Perl氏普鲁士蓝染色，通过图像分析计算含铁色素的面积。全肝切片中铁染色区域的平均百分比为62.52%±21.81%，而活检样本中铁染色区域的平均百分比为71.47%±19.77%。肝脏铁浓度与全切片中铁的百分比（=0.92；<0.0001）、活检样本中铁的百分比（=0.91；<0.0001）以及其他临床病理特征之间存在正相关。通过图像分析评估发现，肝脏活检样本中的含铁血黄素显著高于全肝切片中的含铁血黄素（<0.0001）。
