Liu Sujun, Zhang Jie, Li Daxu, Liu Wei, Luo Xun, Zhang Rongxian, Li Li, Zhao Jian
Leshan Teacher's College, Leshan, 614004, PR China.
Nat Prod Res. 2007 Aug;21(10):915-22. doi: 10.1080/14786410701494686.
Cirsium japonicum DC, a traditional Chinese medicine, has been widely used as an antihemorrhagic and diuretic agent. The objective of this study was to perform quantitative analysis of flavone by reversed-phase HPLC and examine the anticancer activity of C. japonicum DC in the S180 and H22 mice. Cirsium japonicum DC was separated and purified with several chromatography techniques and two flavone compounds, pectolinarin and 5,7-dihydroxy-6,4'-dimethoxyflavone, were isolated. The content of these two compounds in the methanol, ethanol, and aqueous extractions respectively was determined by HPLC as follows: pectolinarin 1.87%, 1.65%, 1.27%; 5,7-dihydroxy-6,4'-dimethoxyflavone: 0.515%, 0.42%, 0.221%. Furthermore, the effect of the two flavones on the anticancer activity in S180 and H22 mice was studied. Our research shows that these two flavones greatly inhibit cancer cell growth. The rate of inhibiting S180 mice was 55.77% at 50 mg kg( - 1), and the rate of life lengthening was 99.13% at 50 mg kg( - 1) in H22 mice.
中药大蓟，一直被广泛用作止血和利尿剂。本研究的目的是通过反相高效液相色谱法对黄酮进行定量分析，并检测大蓟在S180和H22小鼠体内的抗癌活性。采用多种色谱技术对大蓟进行分离纯化，分离得到两种黄酮化合物，即果胶菌素和5,7-二羟基-6,4'-二甲氧基黄酮。通过高效液相色谱法测定这两种化合物在甲醇、乙醇和水提取物中的含量如下：果胶菌素分别为1.87%、1.65%、1.27%；5,7-二羟基-6,4'-二甲氧基黄酮分别为0.515%、0.42%、0.221%。此外，还研究了这两种黄酮对S180和H22小鼠抗癌活性的影响。我们的研究表明，这两种黄酮能显著抑制癌细胞生长。在S180小鼠中，50mg kg(-1)时的抑制率为55.77%，在H22小鼠中，50mg kg(-1)时的延长寿命率为99.13%。
