Blomgen H, Viland H
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
Anticancer Res. 1991 Sep-Oct;11(5):1849-53.
Biostim, which is a glucoprotein extract of Klebsiella pneumoniae, is known to trigger human monocytes to increased secretion of metabolites, which inhibit lymphocyte mitogenesis and which augment NK-activity of lymphocytes. Some aspects of this monocyte activation have been examined in this study. The conclusion of the present investigation is that preincubation of human monocytes for 6-24 h in serum-free medium at 37 degrees C renders them unresponsive to Biostim, as assessed by secretion of the above factors. Monocytes which were preincubated in 100% serum or in serum-free medium at 3 degrees C retained their responsiveness to Biostim. Loss of Biostim responsiveness could not be prevented by interferon alpha, beta, gamma or by granulocyte-monocyte and granulocyte colony stimulating factors. One interpretation of these results is that human monocytes possess distinct receptors for Biostim whose expression is lost under suboptimal culture conditions. Expression of these receptors is a prerequisite for responsiveness to the drug.
生物刺激素是肺炎克雷伯菌的一种糖蛋白提取物，已知它能促使人类单核细胞增加代谢产物的分泌，这些代谢产物可抑制淋巴细胞有丝分裂，并增强淋巴细胞的自然杀伤活性。本研究对这种单核细胞激活的某些方面进行了检测。本调查的结论是，在37℃无血清培养基中将人类单核细胞预孵育6 - 24小时后，根据上述因子的分泌情况评估，它们对生物刺激素无反应。在100%血清中或在3℃无血清培养基中预孵育的单核细胞对生物刺激素仍保持反应性。α、β、γ干扰素或粒细胞 - 单核细胞及粒细胞集落刺激因子均无法阻止生物刺激素反应性的丧失。这些结果的一种解释是，人类单核细胞拥有生物刺激素的独特受体，其表达在次优培养条件下会丧失。这些受体的表达是对该药物产生反应的前提条件。
