Estaquier J, Bloy C, Corallo F, Peron C, Ameisen J C
INSERM U415, Institut Pasteur, Lille, France.
Immunopharmacology. 1998 May;39(2):157-64. doi: 10.1016/s0162-3109(98)00002-2.
Programmed cell death or apoptosis is a physiological cell suicide process that can be suppressed by survival factors. Monocytes undergo rapid apoptosis in culture, unless signalled by cytokines or the bacterial lipopolysaccharide LPS. We have investigated the effect on monocyte apoptosis of the immunostimulating agent RU 41740 (Biostim), a glycoprotein extract from the Klebsiella pneumoniae K2O1 strain that is used for the prevention of recurrent infections. RU 41740, as LPS, strongly enhanced monocyte survival in vitro, an effect related to apoptosis suppression. RU 41740 at concentration ranging from 1 ng/ml to 10 microg/ml prevented apoptosis induced both by survival factor deprival and by gamma-irradiation. Our observation suggests that enhancement of monocyte survival may represent a component of the reported immunostimulating effect of this compound.
程序性细胞死亡或细胞凋亡是一种可被存活因子抑制的生理性细胞自杀过程。单核细胞在培养中会迅速凋亡，除非受到细胞因子或细菌脂多糖LPS的信号刺激。我们研究了免疫刺激剂RU 41740（Biostim）对单核细胞凋亡的影响，RU 41740是一种从肺炎克雷伯菌K2O1菌株中提取的糖蛋白提取物，用于预防反复感染。与LPS一样，RU 41740在体外能显著提高单核细胞的存活率，这种作用与抑制细胞凋亡有关。浓度在1 ng/ml至10 μg/ml范围内的RU 41740可防止因剥夺存活因子和γ射线照射所诱导的细胞凋亡。我们的观察结果表明，单核细胞存活率的提高可能是该化合物所报道的免疫刺激作用的一个组成部分。
