Chiang H C, Tseng T H, Wang C J, Chen C F, Kan W S
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, R.O.C.
Anticancer Res. 1991 Sep-Oct;11(5):1911-7.
beta-Sitosterol (SI-0), beta-sitosterol glucoside (SI-1), dioscin (SI-2), methyl protoprosapogenin A of dioscin (SI-3), methyl protodioscin (SI-4) and protodioscin (SI-5) were isolated and characterized from the whole plant of Solanum indicum L. (Solanaceae). Except for beta-sitosterol, these compounds have not been previously isolated from Solanum indicum L. Both CHCl3 soluble (SI-IV) and insoluble (SI-V) fractions of the ethanolic extract (SI-I) showed cytotoxicity on seven cancer cell lines: Colo-205 (colon), KB (nasopharynx), HeLa (uterine cervix), HA22T (hepatoma), Hep-2 (laryngeal epidermoid), GBM8401/TSGH (glioma) and H1477 (melanoma). The purified constituents, SI-2 and SI-4 showed more potent effects by DEA and MTT assay. SI-2,3,4 and 5 also demonstrated cytotoxicity on cultured C6 glioma cells by PRE assay, ans SI-3,4 and 5 showed a tumor inhibitory effect in vivo in C6 glioma cells. In addition, SI-2 had an inhibitory effect on the DNA synthesis of C6 glioma cells at 10 micrograms/ml.
从茄科植物龙葵的全株中分离并鉴定出了β-谷甾醇（SI-0）、β-谷甾醇葡萄糖苷（SI-1）、薯蓣皂苷（SI-2）、薯蓣皂苷元甲基原皂苷A（SI-3）、甲基原薯蓣皂苷（SI-4）和原薯蓣皂苷（SI-5）。除β-谷甾醇外，这些化合物此前尚未从龙葵中分离得到。乙醇提取物（SI-I）的氯仿可溶部分（SI-IV）和不溶部分（SI-V）对七种癌细胞系均显示出细胞毒性，这七种癌细胞系分别为：Colo-205（结肠癌细胞）、KB（鼻咽癌细胞）、HeLa（子宫颈癌细胞）、HA22T（肝癌细胞）、Hep-2（喉表皮样癌细胞）、GBM8401/TSGH（胶质瘤细胞）和H1477（黑色素瘤细胞）。通过二乙基亚硝胺（DEA）和噻唑蓝（MTT）检测，纯化后的成分SI-2和SI-4显示出更强的作用效果。通过蛋白免疫印迹法（PRE）检测，SI-2、3、4和5对培养的C6胶质瘤细胞也表现出细胞毒性，并且通过体内实验，SI-3、4和5对C6胶质瘤细胞显示出肿瘤抑制作用。此外，SI-2在浓度为10微克/毫升时对C6胶质瘤细胞的DNA合成具有抑制作用。
