Ji J, McDermott J L, Dluzen D E
Department of Anatomy, Northeastern Ohio Universities College of Medicine, 4209 State Route 44, Rootstown, OH 44272, USA.
J Neuroendocrinol. 2007 Sep;19(9):725-31. doi: 10.1111/j.1365-2826.2007.01581.x.
Reserpine inhibits vesicular monoamine transporter-2 (VMAT-2) function and thereby impairs vesicular dopamine (DA) storage within nerve terminals. The present report compared the effects of reserpine treatment upon the striatal dopaminergic system in male and female mice as a means to assess potential sex differences in VMAT-2/DA storage function. After treatment with reserpine, male mice showed significantly greater striatal DA concentrations and K+ -evoked DA output from the striatum compared to females. By contrast, no statistically significant sex differences were observed in methamphetamine-evoked DA output in reserpine-treated mice. These results demonstrate a clear sex difference in the striatal dopaminergic responses to reserpine and suggest that females possess a more active VMAT-2/DA storage capacity, as indicated by the greater degree of deficits observed when VMAT-2/DA storage function is inhibited by reserpine. Such findings have important implications for understanding some of the bases for sex differences in neurotoxicity and neurodegeneration of the nigrostriatal dopaminergic system.
利血平抑制囊泡单胺转运体2（VMAT-2）的功能，从而损害神经末梢内的囊泡多巴胺（DA）储存。本报告比较了利血平处理对雄性和雌性小鼠纹状体多巴胺能系统的影响，以此作为评估VMAT-2/DA储存功能潜在性别差异的一种方法。利血平处理后，与雌性小鼠相比，雄性小鼠纹状体中的DA浓度以及纹状体中K⁺诱发的DA释放量显著更高。相比之下，在利血平处理的小鼠中，甲基苯丙胺诱发的DA释放量未观察到统计学上的显著性别差异。这些结果表明，纹状体对利血平的多巴胺能反应存在明显的性别差异，并且表明雌性具有更活跃的VMAT-2/DA储存能力，这可通过利血平抑制VMAT-2/DA储存功能时观察到的更大程度的缺陷来表明。这些发现对于理解黑质纹状体多巴胺能系统神经毒性和神经退行性变性别差异的一些基础具有重要意义。
