用于检测野油菜黄单胞菌辣椒斑点病致病变种中铜抗性基因的DNA探针。
DNA probes for detection of copper resistance genes in Xanthomonas campestris pv. vesicatoria.
作者信息
Garde S, Bender C L
机构信息
Department of Plant Pathology, Oklahoma State University, Stillwater 74078.
出版信息
Appl Environ Microbiol. 1991 Aug;57(8):2435-9. doi: 10.1128/aem.57.8.2435-2439.1991.
The copper resistance (Cur) genes encoded on pXV10A, a 190-kb plasmid in Xanthomonas campestris pv. vesicatoria XV10, were isolated on a 44-kb cosmid clone designated pCuR1. Tn5 mutagenesis of pCuR1 indicated that a 4.0-kb region was required for copper resistance. Three restriction fragments located within the 4.0-kb region demonstrated high specificity for the Cur genes present in X. campestris pv. vesicatoria and will be useful in monitoring the presence of these genes in the environment.
编码在野油菜黄单胞菌致病变种XV10的190 kb质粒pXV10A上的铜抗性（Cur）基因，在一个名为pCuR1的44 kb黏粒克隆上分离得到。对pCuR1进行Tn5诱变表明，铜抗性需要一个4.0 kb的区域。位于4.0 kb区域内的三个限制性片段对野油菜黄单胞菌致病变种中存在的Cur基因具有高度特异性，将有助于监测环境中这些基因的存在。
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