丁香假单胞菌番茄致病变种铜抗性基因的核苷酸序列与结构
Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. tomato.
作者信息
Mellano M A, Cooksey D A
机构信息
Department of Plant Pathology, University of California, Riverside 92521.
出版信息
J Bacteriol. 1988 Jun;170(6):2879-83. doi: 10.1128/jb.170.6.2879-2883.1988.
Abstract
The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF.
摘要
丁香假单胞菌番茄致病变种中一个4.5千碱基铜抗性决定簇的核苷酸序列显示出四个同向的开放阅读框（ORF）。缺失和位点特异性突变分析表明，前两个ORF对铜抗性至关重要；后两个ORF是完全抗性所必需的，但在没有它们的情况下也能赋予低水平抗性。在第二个ORF开头附近发现了五个高度保守的直接24碱基重复序列，并且在第一个ORF中间发现了一个相似但保守性较低的重复区域。
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