Barbour W M, Hattermann D R, Stacey G
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Appl Environ Microbiol. 1991 Sep;57(9):2635-9. doi: 10.1128/aem.57.9.2635-2639.1991.
The chemotactic response of Bradyrhizobium japonicum toward soybean seed and root exudates was examined. Assays using various isoflavones and fractionated exudate indicated that isoflavones are not the principal attractants in exudates. Likewise, induction of nod genes with isoflavones or seed exudate before assay did not enhance chemotaxis. Screening of numerous compounds revealed that only dicarboxylic acids and the amino acids glutamate and aspartate were strong attractants. The presence of glutamate, aspartate, and dicarboxylic acids in appreciable concentrations in soybean seed and root exudates indicates that these compounds likely represent natural chemoattractants for B. japonicum.
研究了日本慢生根瘤菌对大豆种子和根分泌物的趋化反应。使用各种异黄酮和分级分离的分泌物进行的测定表明,异黄酮不是分泌物中的主要引诱剂。同样,在测定前用异黄酮或种子分泌物诱导结瘤基因并没有增强趋化作用。对众多化合物的筛选表明,只有二羧酸以及氨基酸谷氨酸和天冬氨酸是强引诱剂。大豆种子和根分泌物中存在相当浓度的谷氨酸、天冬氨酸和二羧酸,这表明这些化合物可能是日本慢生根瘤菌的天然化学引诱剂。