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刀豆种子中一种凝集素的结构：对旧分子的新结构见解

Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules.

作者信息

Delatorre Plínio, Rocha Bruno A M, Souza Emmanuel P, Oliveira Taianá M, Bezerra Gustavo A, Moreno Frederico B M B, Freitas Beatriz T, Santi-Gadelha Tatiane, Sampaio Alexandre H, Azevedo Walter F, Cavada Benildo S

机构信息

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Ceará, Brazil.

出版信息

BMC Struct Biol. 2007 Aug 2;7:52. doi: 10.1186/1472-6807-7-52.

DOI:10.1186/1472-6807-7-52
PMID:17683532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1955443/
Abstract

BACKGROUND

Lectins are mainly described as simple carbohydrate-binding proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and isolated amino acid residues. We report the crystal structure of a lectin isolated from Canavalia gladiata seeds (CGL), describing a new binding pocket, which may be related to pathogen resistance activity in ConA-like lectins; a site where a non-protein amino-acid, alpha-aminobutyric acid (Abu), is bound.

RESULTS

The overall structure of native CGL and complexed with alpha-methyl-mannoside and Abu have been refined at 2.3 A and 2.31 A resolution, respectively. Analysis of the electron density maps of the CGL structure shows clearly the presence of Abu, which was confirmed by mass spectrometry.

CONCLUSION

The presence of Abu in a plant lectin structure strongly indicates the ability of lectins on carrying secondary metabolites. Comparison of the amino acids composing the site with other legume lectins revealed that this site is conserved, providing an evidence of the biological relevance of this site. This new action of lectins strengthens their role in defense mechanisms in plants.

摘要

背景

凝集素主要被描述为简单的碳水化合物结合蛋白。先前的研究试图确定其他结合位点，这些位点可能识别植物激素、次生代谢产物和分离的氨基酸残基。我们报道了从刀豆种子中分离出的一种凝集素（CGL）的晶体结构，描述了一个新的结合口袋，它可能与伴刀豆球蛋白样凝集素的病原体抗性活性有关；这是一个结合了非蛋白质氨基酸α-氨基丁酸（Abu）的位点。

结果

天然CGL以及与α-甲基甘露糖苷和Abu复合的CGL的整体结构分别在2.3 Å和2.31 Å分辨率下得到了优化。对CGL结构的电子密度图分析清楚地显示了Abu的存在，这通过质谱得到了证实。

结论

植物凝集素结构中Abu的存在有力地表明了凝集素携带次生代谢产物的能力。将构成该位点的氨基酸与其他豆科凝集素进行比较，发现该位点是保守的，这为该位点的生物学相关性提供了证据。凝集素的这种新作用强化了它们在植物防御机制中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/2aa5a9b2e479/1472-6807-7-52-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/5b7154550ee8/1472-6807-7-52-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/14e45cc35f02/1472-6807-7-52-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/e081fe18fae6/1472-6807-7-52-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/0eb73d37fd92/1472-6807-7-52-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/5ceb367c5ed9/1472-6807-7-52-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/2aa5a9b2e479/1472-6807-7-52-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/5b7154550ee8/1472-6807-7-52-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/14e45cc35f02/1472-6807-7-52-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/e081fe18fae6/1472-6807-7-52-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/0eb73d37fd92/1472-6807-7-52-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/5ceb367c5ed9/1472-6807-7-52-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d06b/1955443/2aa5a9b2e479/1472-6807-7-52-6.jpg

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