Liu Jie, Wang Ji-Yao, Wei Li-Ming, Lu Ye, Jin Hong
Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhonghua Yi Xue Za Zhi. 2007 May 15;87(18):1272-5.
To study the effects of Fuzheng Huayu Decoction on plasma proteome in cirrhosis.
Twenty-six male S-D rats were randomly divided into three groups, cirrhotic model group (n = 10), treated with CCl4 (CCl4/olive oil: v/v = 1:1), Fuzheng Huayu Decoction intervention group (n = 10), treated with CCl4 + Fuzheng Huayu Decoction, and normal control group (n = 6), treated with olive oil only. After 8 weeks, blood sample was collected from the vena cava inferior to undergo bi-dimensional electrophoresis (2DE) and analysis by PDQuest 7.3 software. Differential protein spots were cut, enzyme hydrolysis was conducted, and peptide fragments extracted from the mixture underwent mass spectrometry with MALDI-TOF-TOF-MS. The liver fibrogenesis was assessed by digital image analysis instrument of Masson's trichrome stained sections.
The fibrosis area of the Fuzheng Huayu Decoction was 9% +/- 4%, significantly smaller than that of the cirrhotic model group (12% +/- 5%, P < 0.05). Ten markedly changed protein spots were identified by MALDI-TOF-TOF-MS. Eight of the 10 proteins, including plasma glutathione peroxidase, plasma glutathione peroxidase precursor, prealbumin, haptoglobin, apolipoprotein A-IV precursor, complement C4, inter-alpha-inhibitor H4 heavy chain, and serine/threonine-protein kinase MARK1 (microtubule- affinity regulating kinase 1) were expressed very lowly in the cirrhotic model group while were expressed highly in the Fuzheng Huayu Decoction group. The expression of liver regeneration-related protein LRRG03 and vimentin increased in the cirrhotic model group, and reduced in the Fuzheng Huayu Decoction group.
Some proteins related to oxidative stress, cell proliferation and transformation have changed in the plasma of cirrhosis induced by CCl4. Fuzheng Huayu Decoction promotes protein synthesis and plays an anti-fibrotic role by antioxidation and accommodation of cell proliferation and transformation.
研究扶正化瘀方对肝硬化大鼠血浆蛋白质组的影响。
将26只雄性SD大鼠随机分为三组，肝硬化模型组（n = 10），以四氯化碳（CCl4/橄榄油：v/v = 1:1）处理；扶正化瘀方干预组（n = 10），以四氯化碳 + 扶正化瘀方处理；正常对照组（n = 6），仅以橄榄油处理。8周后，从下腔静脉采集血样，进行双向电泳（2DE），并用PDQuest 7.3软件分析。切下差异蛋白点，进行酶解，从混合物中提取的肽段用基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF-TOF-MS）进行质谱分析。用Masson三色染色切片的数字图像分析仪评估肝纤维化程度。
扶正化瘀方组的纤维化面积为9%±4%，显著小于肝硬化模型组（12%±5%，P < 0.05）。通过MALDI-TOF-TOF-MS鉴定出10个明显变化的蛋白点。10种蛋白质中的8种，包括血浆谷胱甘肽过氧化物酶、血浆谷胱甘肽过氧化物酶前体、前白蛋白、触珠蛋白、载脂蛋白A-IV前体、补体C4、α-抑制因子H4重链和丝氨酸/苏氨酸蛋白激酶MARK1（微管亲和调节激酶1）在肝硬化模型组中表达极低，而在扶正化瘀方组中表达较高。肝硬化模型组中肝再生相关蛋白LRRG03和波形蛋白的表达增加，而在扶正化瘀方组中降低。
四氯化碳诱导的肝硬化大鼠血浆中一些与氧化应激、细胞增殖和转化相关的蛋白质发生了变化。扶正化瘀方通过抗氧化以及调节细胞增殖和转化促进蛋白质合成，发挥抗纤维化作用。
