Hano Takeshi, Oshima Yuji, Kim Sang Gyoon, Satone Hina, Oba Yumi, Kitano Takeshi, Inoue Suguru, Shimasaki Yohei, Honjo Tsuneo
Division of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Chemosphere. 2007 Oct;69(6):927-33. doi: 10.1016/j.chemosphere.2007.05.093. Epub 2007 Aug 7.
We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).
我们研究了三丁基锡（TBT）对日本青鳉（Oryzias latipes）胚胎发育、孵化成功率和性别分化的影响。将受精后8小时内的胚胎通过纳米注射在卵内暴露于浓度为0（对照）、0.16、0.80、3.96、19.2和82.1纳克/卵的TBT中。观察胚胎的存活、发育和孵化情况。孵化出的幼鱼饲养至60天性成熟，根据DMY（青鳉中的一个雄性决定基因）的存在和第二性征，依据遗传和表型性别来检查性别分化情况。结果显示，TBT导致了浓度依赖性死亡并损害了胚胎发育。然而，在60日龄的青鳉成鱼中未检测到雄性化现象。诱导胚胎发育异常的最低观察有效浓度估计为0.16纳克/卵（约160纳克/克卵）。
