Shi Yanhong, Chen Chen, Li Ming, Liu Lei, Dong Kejun, Chen Kun, Qiu Xuchun
Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, China.
Antioxidants (Basel). 2021 Oct 20;10(11):1647. doi: 10.3390/antiox10111647.
The widely used compound tributyltin (TBT), which can be continuously detected in aquatic species and seafood, may induce diverse adverse effects on aquatic organisms. However, little is known regarding the mechanistic links between behavioral abnormality and oxidative stress in different fish tissues in response to oral TBT exposure. Herein, juvenile Japanese medaka () were orally exposed to TBT at 1 and 10 ng/g-bw/d for four weeks. After exposure, the locomotor activity and social interaction of juvenile medaka were found to be significantly reduced in the 10 ng/g-bw/d TBT-exposed group. Furthermore, the antioxidant biomarkers in different tissues of juvenile medaka showed different levels of sensitivity to TBT exposure. The eye superoxide dismutase (SOD) activities markedly increased in both groups exposed to 1 and 10 ng/g-bw/d TBT, while the eye and brain malondialdehyde (MDA) levels increased in the higher dose group. Furthermore, the eye and brain ATPase activities markedly declined in the 1 ng/g-bw/d TBT-exposed group. A correlation analysis revealed that the decreased locomotor activity and social interaction in medaka were associated with the eye antioxidant enzyme (i.e., SOD and catalase (CAT)) activity and brain oxidative damage level. Thus, our findings suggested that there might be some mechanistic links between the behavioral abnormality induced by TBT exposure and oxidative stress in the eyes and brains of medaka. Thus, our findings indicate that the impacts of oral exposure to TBT should be considered to better assess its risk to the aquatic ecosystem and human health.
广泛使用的化合物三丁基锡（TBT）可在水生物种和海产品中持续检测到，它可能会对水生生物产生多种不利影响。然而，关于口服TBT暴露后不同鱼类组织中行为异常与氧化应激之间的机制联系，我们知之甚少。在此，将日本青鳉幼鱼以1和10 ng/g体重/天的剂量口服暴露于TBT中四周。暴露后，发现10 ng/g体重/天TBT暴露组的日本青鳉幼鱼的运动活性和社交互动显著降低。此外，日本青鳉幼鱼不同组织中的抗氧化生物标志物对TBT暴露表现出不同程度的敏感性。在暴露于1和10 ng/g体重/天TBT的两组中，眼睛超氧化物歧化酶（SOD）活性均显著增加，而在高剂量组中，眼睛和大脑丙二醛（MDA）水平升高。此外，在1 ng/g体重/天TBT暴露组中，眼睛和大脑的ATP酶活性显著下降。相关性分析表明，青鳉运动活性和社交互动的降低与眼睛抗氧化酶（即SOD和过氧化氢酶（CAT））活性以及大脑氧化损伤水平有关。因此，我们的研究结果表明，TBT暴露诱导的行为异常与青鳉眼睛和大脑中的氧化应激之间可能存在一些机制联系。因此，我们的研究结果表明，应考虑口服TBT暴露的影响，以便更好地评估其对水生生态系统和人类健康的风险。
