Bhanja Payel, Sengupta Satarupa, Banerjee Debjani, Sarkar Kamalesh, Jana Smarajit, Chakrabarti Sekhar
HIV/AIDS Laboratory, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Calcutta, India.
Virus Res. 2007 Dec;130(1-2):310-4. doi: 10.1016/j.virusres.2007.06.013. Epub 2007 Aug 7.
HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C with respect to both env and nef genes; however, 17 samples (43.6%) showed distinct subtype discordance. Simplot analysis of these samples showed the presence of intersubtype recombination between subtypes C and B. Both env C/nef B and env B/nef C recombinants were found to be present; 16 samples were found to be env C/nef B and 1 sample was detected as env B/nef C. This result indicates the emergence of intersubtype recombinants of HIV-1 for the first time in this eastern part of India.
对印度加尔各答女性性工作者中检测出的HIV-1进行了env和nef基因特征分析。该研究共使用了39份HIV-1血清阳性样本。对各区域核苷酸序列的系统发育分析表明，39份样本中有22份（56.4%）在env和nef基因方面均感染了C亚型；然而，17份样本（43.6%）表现出明显的亚型不一致。对这些样本的Simplot分析显示C亚型和B亚型之间存在亚型间重组。发现同时存在env C/nef B和env B/nef C重组体；16份样本为env C/nef B，1份样本检测为env B/nef C。这一结果表明在印度东部首次出现了HIV-1亚型间重组体。
