Schlaphoff Verena, Klade Christoph S, Jilma Bernd, Jelovcan Sandra B, Cornberg Markus, Tauber Erich, Manns Michael P, Wedemeyer Heiner
Hannover Medical School, Department of Gastroenterology, Hepatology and Endocrinology, Carl-Neuberg Str. 1, 30625 Hannover, Germany.
Vaccine. 2007 Sep 17;25(37-38):6793-806. doi: 10.1016/j.vaccine.2007.06.026. Epub 2007 Jun 29.
Only very limited information on phenotype and function of vaccine-induced CD8+ T cells is available for humans. We investigated hepatitis C virus-specific CD8+ T cells after vaccination with the HCV peptide-vaccine IC41 which includes 5 MHC-class I and 3 MHC class-II-restricted epitopes. In healthy subjects, IC41 induced both HCV-specific central memory as well as effector CD8+ T cells which rapidly expanded upon antigen exposure in vitro. IFNgamma production was dependent on formulation of the synthetic peptides with the adjuvant poly-l-arginine. In chronic HCV patients, the frequency of HCV-specific CD8+ T cells increased after vaccination with a decline of CD45RA-positive effector memory cells in some but not all patients. Thus, this study suggests that HCV-specific memory cells can be induced by peptide vaccination and that a reversion of functional impaired phenotypes by therapeutic vaccination is possible in chronic HCV infection.
关于疫苗诱导的人类CD8+T细胞的表型和功能，目前仅有非常有限的信息。我们研究了接种丙型肝炎病毒（HCV）肽疫苗IC41后的HCV特异性CD8+T细胞，该疫苗包含5个MHC-I类和3个MHC-II类限制性表位。在健康受试者中，IC41诱导产生了HCV特异性中枢记忆细胞以及效应性CD8+T细胞，这些细胞在体外接触抗原后迅速扩增。γ干扰素的产生取决于合成肽与佐剂聚-L-精氨酸的配方。在慢性HCV患者中，接种疫苗后HCV特异性CD8+T细胞的频率增加，部分但并非所有患者的CD45RA阳性效应记忆细胞数量减少。因此，本研究表明肽疫苗接种可诱导HCV特异性记忆细胞，并且在慢性HCV感染中，通过治疗性疫苗接种有可能逆转功能受损的表型。
